Normalization and analysis of cDNA microarrays using within-array replications applied to neuroblastoma cell response to a cytokine

Jianqing Fan Paul Tam George Vande Woude Yi Ren

Data Analysis mathscidoc:1912.43303

Proceedings of the National Academy of Sciences, 101, (5), 1135-1140, 2004.2
The quantitative comparison of two or more microarrays can reveal, for example, the distinct patterns of gene expression that define different cellular phenotypes or the genes that are induced in the cellular response to certain stimulations. Normalization of the measured intensities is a prerequisite of such comparisons. However, a fundamental problem in cDNA microarray analysis is the lack of a common standard to compare the expression levels of different samples. Several normalization protocols have been proposed to overcome the variabilities inherent in this technology. We have developed a normalization procedure based on within-array replications via a semilinear in-slide model, which adjusts objectively experimental variations without making critical biological assumptions. The significant analysis of gene expressions is based on a weighted <i>t</i> statistic, which accounts for the heteroscedasticity of the
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@inproceedings{jianqing2004normalization,
  title={Normalization and analysis of cDNA microarrays using within-array replications applied to neuroblastoma cell response to a cytokine},
  author={Jianqing Fan, Paul Tam, George Vande Woude, and Yi Ren},
  url={http://archive.ymsc.tsinghua.edu.cn/pacm_paperurl/20191221113605660096863},
  booktitle={Proceedings of the National Academy of Sciences},
  volume={101},
  number={5},
  pages={1135-1140},
  year={2004},
}
Jianqing Fan, Paul Tam, George Vande Woude, and Yi Ren. Normalization and analysis of cDNA microarrays using within-array replications applied to neuroblastoma cell response to a cytokine. 2004. Vol. 101. In Proceedings of the National Academy of Sciences. pp.1135-1140. http://archive.ymsc.tsinghua.edu.cn/pacm_paperurl/20191221113605660096863.
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