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A novel function for dendritic cell: clearance of VEGF via VEGF receptor-1

Yi Xie Jianqing Fan Juhua Chen Fang-Ping Huang Brian Cao Paul KH Tam Yi Ren

Data Analysis mathscidoc:1912.43402

Biochemical and biophysical research communications, 380, (2), 243-248, 2009.3
It has been reported that the plasma levels of VEGF in tumor patients decreased during dendritic cell (DC)-based immunotherapy, but the underlying mechanism remains unclear. Our current report demonstrates that VEGF levels were significantly decreased in the supernatants of DCs incubated with rhVEGF or tumor conditioned medium (TCM) while the intracellular VEGF in DCs was increased. The increased intracellular VEGF was not due to the <i>de novo</i> VEGF synthesis by DCs because exogenous VEGF inhibited the mRNA expression of VEGF in DCs. More direct evidence was provided to demonstrate that Cy3-labeled VEGF could be internalized by DCs specifically and efficiently. In addition, the activity of DCs to internalize VEGF was abolished by neutralizing antibody against VEGF receptor-1 (Flt-1) and inhibitors of endocytosis such as carbonyl cyanide m-chlorophenyl hydrazone (CCCP) and genistein. This
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@inproceedings{yi2009a,
  title={A novel function for dendritic cell: clearance of VEGF via VEGF receptor-1},
  author={Yi Xie, Jianqing Fan, Juhua Chen, Fang-Ping Huang, Brian Cao, Paul KH Tam, and Yi Ren},
  url={http://archive.ymsc.tsinghua.edu.cn/pacm_paperurl/20191221114215021862962},
  booktitle={Biochemical and biophysical research communications},
  volume={380},
  number={2},
  pages={243-248},
  year={2009},
}
Yi Xie, Jianqing Fan, Juhua Chen, Fang-Ping Huang, Brian Cao, Paul KH Tam, and Yi Ren. A novel function for dendritic cell: clearance of VEGF via VEGF receptor-1. 2009. Vol. 380. In Biochemical and biophysical research communications. pp.243-248. http://archive.ymsc.tsinghua.edu.cn/pacm_paperurl/20191221114215021862962.
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