Single-cell (sc)RNA-seq, together with RNA velocity and metabolic labeling, reveals cellular states and transitions at unprecedented resolution. Fully exploiting these data, however, requires kinetic models capable of unveiling governing regulatory functions. Here, we introduce an analytical framework dynamo (https://github.com/aristoteleo/dynamo-release), which infers absolute RNA velocity, reconstructs continuous vector fields that predict cell fates, employs differential geometry to extract underlying regulations, and ultimately predicts optimal reprogramming paths and perturbation outcomes. We highlight dynamo’s power to overcome fundamental limitations of conventional splicing-based RNA velocity analyses to enable accurate velocity estimations on a metabolically labeled human hematopoiesis scRNA-seq dataset. Furthermore, differential geometry analyses reveal mechanisms driving early megakaryocyte appearance and elucidate asymmetrical regulation within the PU.1-GATA1 circuit. Leveraging the least-action-path method, dynamo accurately predicts drivers of numerous hematopoietic transitions. Finally, in silico perturbations predict cell-fate diversions induced by gene perturbations. Dynamo, thus, represents an important step in advancing quantitative and predictive theories of cell-state transitions.

Contrast to the hyperbolic mean curvature flows studied in [HKL], [LS], and [KW], a new hyperbolic curvature flow is proposed for convex hypersurfaces. This flow is most suited when the Gauss curvature is involved. The equation satisfied by the graph of the hypersurface under this flow gives rise to a new class of fully nonlinear Euclidean invariant hyperbolic equations.

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3D shape classification plays a fundamental role in geometric big data analysis. This work proposes a novel method for shape classification based on optimal mass transportation theory. The Riemann surfaces are mapped onto canonical domains conformally based on uniformization theorem. The conformal factor function is treated as probability distribution on the canonical domain. For each pair of probability distributions, the optimal mass transportation map is computed by solving Monge-Amper´e equation. The transportation cost is theWasserstein distance between two distributions. By using this distance, geometric classification based on clustering can be performed. The method is applied to 3D human facial expression recognition, which demonstrates the efficiency and efficacy of the method.

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