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Let M be a complete Riemannian manifold with Riemannian volume volg and f be a smooth function on M. A sharp upper bound estimate on the first eigenvalue of symmetric diffusion operator 􏰂 f = 􏰂 − ∇ f · ∇ was given by Wu (J Math Anal Appl 361:10–18, 2010) and Wang (Ann Glob Anal Geom 37:393–402, 2010) under a condition that finite dimensional Bakry–Émery Ricci curvature is bounded below, independently. They propounded an open problem is whether there is some rigidity on the estimate. In this note, we will solve this problem to obtain a splitting type theorem, which generalizes Li–Wang’s result in (J Differ Geom 58:501–534, 2001, J Differ Geom 62:143–162, 2002). For the case that infinite dimensional Bakry–Emery Ricci curvature of M is bounded below, we donotexpectanyupperboundestimateonthefirsteigenvalueof􏰂f withoutanyadditional assumption (see the example in Sect. 2). In this case, we will give a sharp upper bound estimate on the first eigenvalue of􏰂f under the additional assuption that |∇f| is bounded.We also obtain the rigidity result on this estimate, as another Li–Wang type splitting theorem.

This paper gives a classification of first order polynomial differential operators of form $\mathscr{X} = X_1(x_1,x_2)\delta_1 + X_2(x_1,x_2)\delta_2$, $(\delta_i = \partial/\partial x_i)$. The classification is given through the order of an operator that is defined in this paper. Let $X=\mathscr{X}y$ to be the differential polynomial associated with $\mathscr{X}$, the order of $\mathscr{X}$, $\mathrm{ord}(\mathscr{X})$, is defined as the order of a differential ideal $\Lambda$ of differential polynomials that is a nontrivial expansion of the ideal $\{X\}$ and with the lowest order. In this paper, we prove that there are only four possible values for the order of a differential operator, $0$, $1$, $2$, $3$, or $\infty$. Furthermore, when the order is finite, the expansion $\Lambda$ is generated by $X$ and a differential polynomial $A$, which can be obtained through a rational solution of a partial differential equation that is given explicitly in this paper. When the order is infinite, the expansion $\Lambda$ is just the unit ideal. In additional, if, and only if, the order of $\mathscr{X}$ is $0$, $1$, or $2$, the polynomial differential equation associating with $\mathscr{X}$ has Liouvillian first integrals. Examples and connections with Godbilon-Vey sequences are also discussed.

In this paper, we focus on the stochastic inverse eigenvalue problem of reconstructing a stochastic matrix from the prescribed spectrum. We directly reformulate the stochastic inverse eigenvalue problem as a constrained optimization problem over several matrix manifolds to minimize the distance between isospectral matrices and stochastic matrices. Then we propose a geometric Polak–Ribi`ere–Polyak-based nonlinear conjugate gradient method for solving the constrained optimization problem. The global convergence of the proposed method is established. Our method can also be extended to the stochastic inverse eigenvalue problem with prescribed entries. An extra advantage is that our models yield new isospectral flow methods. Finally, we report some numerical tests to illustrate the efficiency of the proposed method for solving the stochastic inverse eigenvalue problem and the case of prescribed entries.

Zika virus (ZIKV) is a mosquito-borne flavivirus. It was first isolated from Uganda in 1947 and has become an emergent event since 2007. However, because of the inconsistency of alignment methods, the evolution of ZIKV remains poorly understood. In this study, we first use the complete protein and an alignment-free method to build a phylogenetic tree of 87 Zika strains in which Asian, East African, and West African lineages are characterized. We also use the NS5 protein to construct the genetic relationship among 44 Zika strains. For the first time, these strains are divided into two clades: African 1 and African 2. This result suggests that ZIKV originates from Africa, then spread to Asia, Pacific islands, and throughout the Americas. We also perform the phylogeny analysis for 53 viruses in genus Flavivirus to which ZIKV belongs using complete proteins. Our conclusion is consistent with the classification by the hosts and transmission vectors.