We establish a new on-diagonal lower estimate of continuous-time heat kernels for large time on graphs. To achieve the goal, we first introduce an upper estimate of heat kernels in natural graph metric, then use the upper estimate and the volume growth condition to show the validity of the on-diagonal lower estimate.

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We prove the so-called$unitary isotropy theorem$, a result on isotropy of a unitary involution. The analogous previously known results on isotropy of orthogonal and symplectic involutions as well as on hyperbolicity of orthogonal, symplectic, and unitary involutions are formal consequences of this theorem. A component of the proof is a detailed study of the quasi-split unitary grassmannians.

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