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In this paper we prove a new matrix Li-Yau-Hamilton (LYH) estimate for K¨ahler-Ricci flow on manifolds with nonnegative bi- sectional curvature. The form of this new LYH estimate is obtained by the interpolation consideration originated in [Ch] by Chow. This new inequality is shown to be connected with Perelman’s entropy formula through a family of differential equalities. In the rest of the paper, we show several applications of this new estimate and its corresponding estimate for linear heat equation. These include a sharp heat kernel comparison theorem, generalizing the earlier result of Li and Tian [LT], a manifold version of Stoll’s theorem [St] on the characterization of ‘algebraic divisors’, and a localized monotonicity formula for analytic subvari- eties, which sharpens the Bishop volume comparison theorem. Motivated by the connection between the heat kernel estimate and the reduced volume monotonicity of Perelman [P], we prove a sharp lower bound of the fundamental solution to the forward conjugate heat equation, which in a certain sense dual to Perelman’s monotonicity of the reduced volume. As an application of this new monotonicity formula, we show that the blow-down limit of a certain type of long-time solution is a gradient expanding soliton, generalizing an earlier result of Cao. We also illustrate the connection between the new LYH estimate and the Hessian comparison theorem of [FIN] on the forward reduced distance. Localized monotonicity formulae on entropy and forward reduced volume are also derived.

H. Hopf showed that the only constant mean curvature sphere S2 immersed in R3 is the round sphere. The K¨ahler framework is an adequate approach to generalize Hopf’ s theorem to higher dimensions. When ϕ : M → Rn is an isometric immersion from a K¨ahler manifold, the complexified second fundamental form α splits according to types. The (1, 1) part of the second fundamental form plays the role of the mean curvature for surfaces and will be called the pluri-mean curvature pmc. Therefore isometric immersions with parallel pluri-mean curvature (ppmc isometric immersions) generalize in a natural way the cmc immersions. It is a standard fact that R8 is the smallest space where CP2 can be embedded. The aim of this work is to generalize Hopf’s theorem proving in particular that the only ppmc isometric immersion from CP2 into R8 is the standard immersion.