Next-generation sequencing technology enables the routine detection of bacterial pathogens for clinical diagnostics and genetic research. Whole-genome sequencing has been of importance in the epidemiologic analysis of bacterial pathogens. However, few whole-genome sequencing-based genotyping pipelines are available for practical applications. Here, we present the whole-genome sequencing-based single nucleotide polymorphism(SNP) genotyping method and apply to the evolutionary analysis of methicillin-resistant Staphylococcus aureus. The SNP genotyping method calls genome variants using next-generation sequencing reads of whole genomes and calculates the pair-wise Jaccard distances of the genome variants. The method may reveal the high-resolution whole-genome SNP profiles and the structural variants of different isolates of methicillin-resistant S. aureus(MRSA) and methicillin-susceptible S. aureus(MSSA) strains. The phylogenetic analysis of whole genomes and particular regions may monitor and track the evolution and the transmission dynamic of bacterial pathogens. The computer pro- grams of the whole genome sequencing-based SNP genotyping methods are available to the public at https://github. com/ cyinbox/NGS.

Some filtrations of the tensor product of a highest weight module and a lowest weight module over quantum group Uq(g)are constructed in[1]and one can use them to define a two-sided ideal of the modified quantized enveloping algebra. It is shown that the quotient algebra inherits a canonical basis from the modified quantized enveloping algebra and is dual to the quantum coordinate algebra defined by Kashiwara for a symmetrizable Kac–Moody algebra g.

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In this paper, we first establish an $L^2$-type Dolbeault isomorphism for logarithmic differential forms by H\"{o}rmander's $L^2$-estimates. By using this isomorphism and the construction of smooth Hermitian metrics, we obtain a number of new %Akizuki-Kodaira-Nakano type vanishing theorems for sheaves of logarithmic differential forms on compact K\"ahler manifolds with simple normal crossing divisors, which generalize several classical vanishing theorems, including Norimatsu's vanishing theorem, Gibrau's vanishing theorem, Le Potier's vanishing theorem and a version of the Kawamata-Viehweg vanishing theorem.

Denote by Mnthe set of n ×ncomplex matrices. Let f:Mn→[0, ∞)be a continuous map such that f(μUAU∗) =f(A)for any complex unit μ, A ∈Mnand unitary U∈Mn, f(X) =0if and only if X=0and the induced map t →f(tX)is monotonically increasing on [0, ∞)for any rank onenilpotent X∈Mn. Characterization is given for surjective maps φon Mnsatisfying f(AB−BA) =f(φ(A)φ(B) −φ(B)φ(A)). The general theorem isthen used to deduce results on special cases when the function is the pseudo spectrum and the pseudo spectral radius.