In this paper, we prove that discrete Morse functions on digraphs are flat Witten-Morse functions and Witten complexes of transitive digraphs approach to Morse complexes. We construct a chain complex consisting of the formal linear combinations of paths which are not only critical paths of the transitive closure but also allowed elementary paths of the digraph, and prove that the homology of the new chain complex is isomorphic to the path homology. On the basis of the above results, we give the Morse inequalities on digraphs.

We introduce a homotopy theory of digraphs (directed graphs) and prove its basic properties, including the relations to the homology theory of digraphs constructed by the authors in previous papers. In particular, we prove the homotopy invariance of homologies of digraphs and the relation between the fundamental group of the digraph and its first homology group. The category of (undirected) graphs can be identified by a natural way with a full subcategory of digraphs. Thus we obtain also consistent homology and homotopy theories for graphs.

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The spectral radius of a graph is the largest eigenvalue of the adjacency matrix of the graph. Let T(n,D
l) be the tree which minimizes the spectral radius of all trees of order n with exactly l vertices of maximum degree D
. In this paper, T(n,D
l) is determined for D=3, and for l<= 3 and n large enough. It is proven that for sufficiently large n, T(n, 3, l) is a caterpillar with (almost) uniformly distributed legs, T(n,D
2) is a dumbbell, and T(n,
D,3) is a tree consisting of three distinct stars of order D
connected by three disjoint paths of (almost) equal length from their centers to a common vertex. The unique tree with the largest spectral radius among all such trees is also determined. These extend earlier results of Lov´asz and Pelik´an, Simi´c and To˘si´c, Wu, Yuan and Xiao, and Xu, Lin and Shu.

Let $f(n)$ be the maximum number of edges in a graph on $n$ vertices in which no two cycles have the same length. In 1975, Erdos raised the problem of determining $f(n)$ (see J.A. Bondy and U.S.R. Murty, Graph Theory with Applications (Macmillan, New York, 1976), p.247, Problem 11). Given a graph $H$, what is the maximum number of edges of a graph with $n$ vertices not containing $H$ as a subgraph? This number is denoted $ex(n,H)$, and is known as the Turan number. Erdos conjectured that there exists a positive constant $c$ such that $ex(n,C_{2k})\geq cn^{1+1/k}$(see P. Erdos, Some unsolved problems in graph theory and combinatorial analysis, Combinatorial Mathematics and its Applications (Proc. Conf., Oxford, 1969) , pp. 97--109, Academic Press, London, 1971). Haj\'{o}s conjectured that every simple even graph on $n$ vertices can be decomposed into at most $n/2$ cycles (see L. Lovasz, On covering of graphs, in: P. Erdos, G.O.H. Katona (Eds.), Theory of Graphs, Academic Press, New York, 1968, pp. 231 - 236 ). We present the problems, conjectures related to these problems and we summarize the know results.