Given a Lagrangian sphere in a symplectic 4–manifold $(M, \omega)$ with $b^{+}=1$, we find embedded symplectic surfaces intersecting it minimally. When the Kodaira dimension  $\kappa$ of $(M,\omega)$ is $-\infty$, this minimal intersection property turns out to be very powerful for both the uniqueness and existence problems of Lagrangian spheres. On the uniqueness side, for a symplectic rational manifold and any class which is not characteristic, we show that homologous Lagrangian spheres are smoothly isotopic, and when the Euler number is less than 8, we generalize Hind and Evans’ Hamiltonian uniqueness in the monotone case. On the existence side, when  $\kappa=-\infty$, we give a characterization of classes represented by Lagrangian spheres, which enables us to describe the non-Torelli part of the symplectic mapping class group.

In this paper, we first provide an explicit description of {\it all} holomorphic discs (``disc instantons'') attached to Lagrangian torus fibers of arbitrary compact toric manifolds, and prove their Fredholm regularity. Using this, we compute Fukaya-Oh-Ohta-Ono's (FOOO's) obstruction (co) chains and the Floer cohomology of Lagrangian torus fibers of Fano toric manifolds. In particular specializing to the formal parameter T^{2}= e^{-1} , our computation verifies the folklore that FOOO's obstruction (co) chains correspond to the Landau-Ginzburg superpotentials under the mirror symmetry correspondence, and also proves the prediction made by K. Hori about the Floer cohomology of Lagrangian torus fibers of Fano toric manifolds. The latter states that the Floer cohomology (for the parameter value T^{2}= e^{-1} ) of all the fibers vanish except at a finite number, the Euler characteristic of the toric manifold, of base points in the momentum polytope that are critical points of the superpotential of the Landau-Ginzburg mirror to the toric manifold. In the latter cases, we also prove that the Floer cohomology of the corresponding fiber is isomorphic to its singular cohomology.

We introduce new boundary conditions for differential forms on symplectic manifolds with boundary. These boundary conditions, dependent on the symplectic structure, allows us to write down elliptic boundary value problems for both second-order and fourth-order symplectic Laplacians and establish Hodge theories for the cohomologies of primitive forms on manifolds with boundary. We further use these boundary conditions to define a relative version of the primitive cohomologies and to relate primitive cohomologies with Lefschetz maps on manifolds with boundary. As we show, these cohomologies of primitive forms can distinguish certain Kahler structures of Kahler manifolds with boundary.

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We make the elementary observation that the Lagrangian submanifolds of$C$^{$n$},$n$≥3, constructed by Ekholm, Eliashberg, Murphy and Smith are non-uniruled and, moreover, have infinite relative Gromov width. The construction of these submanifolds involve exact Lagrangian caps, which obviously are non-uniruled in themselves. This property is also used to show that if a Legendrian submanifold inside a contactisation admits an exact Lagrangian cap, then its Chekanov–Eliashberg algebra is acyclic.